WebMar 21, 2024 · Suppression of wild-type IDH2 by shRNA resulted in elevated α-KG and decreased isocitrate/citrate, leading to reduced lipid synthesis, a significant decrease in c-Myc downregulated by α-KG, and an inhibition of AML viability and proliferation. ... After 72 h, the infected cells were selected with 0.5 mg/mL puromycin (Selleck). Small ... WebMay 30, 2024 · Hydrocortisone / pramoxine topical Pregnancy Warnings. This drug should be used during pregnancy only if the benefit outweighs the risk to the fetus. -Do not use …
Lentivirus shRNA, plasmids and lentiviral particle OriGene
WebJun 1, 2024 · Objective: To identify candidate shRNA that can knockdown CD3 and achieve inhibition of TCR signaling in primary human T cells. Methods: Peripheral blood derived T cells from healthy donors were transduced with the appropriate vector then subjected to puromycin selection for further 48 hours after transduction. On day 8, cells were finally ... Webpuromycin resistance gene. F. To select for infected cells, add puromycin to the media at the concentration determined in step E.2. Addgene recommends that you maintain one … cf1 study
Are you familiar with the puromycin concentration in HEK …
WebTRC pLKO.1: Go to Millipore Sigma. Search for your gene of interest, scroll until you find the gene information box which includes links for products and click on “shRNA”. Click pricing for “Bacterial Glycerol Stock” for either the human or mouse for the list of available clones in the Sigma library. Use the TRCN# to search the Vector ... WebC. Dilute puromycin in the preferred culture media for your target cells. The final concentration of puromycin should be from 1-10 μg/mL in 1 μg/mL increments. D. Label plates from 1-10 and add appropriate puromycin-containing media to cells. Days 3+: E. Examine cells each day and change to fresh puromycin-containing media every other day. F. http://www.hollingscancercenter.org/research/shared-resources/shRNA/Lentivirusproduction-shRNA.pdf cf1 training