WebThe Incucyte ® Cell Cycle Green/Red Lentivirus Reagent is a fluorescent, single cassette indicator expressing both the GFP (green fluorescent protein) and mKate2 (red fluorescent protein) to distinguish between cells in the G1 and S/G2/M cell cycle phase without altering cell function.. This reagent is ideal for generating stable cell populations or clones using … WebMay 6, 2024 · Cytolight Rapid Red Dye (0.33 µM) or CSFE (1:100 dilution, ThermoFisher). Cells were incubated for 20 min at 37°C and washed 2x with medium supplemented with serum. A total of 5x104 were co-cultured with macrophages in a 96-well plate and incubated for 3h at 37°C and 5% CO 2. After 3h of incubation AML cells were gently removed by
Incucyte® Nuclight Reagents Cell Proliferation - Essen
WebThe IncuCyte NucLight Rapid Red Reagent for nuclear labeling is a cell permeable DNA stain that specifically stains nuclei in cells and is ideally suited to the mix-and-read, real-time quantification using the IncuCyte® Live-Cell Analysis System. More information IncuCyte® ClearView 96-well Chemotaxis Plate WebFeatures: Efficient, non-perturbing nuclear labeling of live mammalian cells for cell counting Homogenous expression of a nuclear-restricted mKate2 (red fluorescent protein) Minimal toxicity, no alteration of cell function Ideal for generating stable cell populations or clones using puromycin selection curfew during covid 19 in south africa
Supplementary Methods M2-polarized macrophages control …
WebIncucyte ® CytoLight Rapid Dyes for live-cell labelling enable simple, fast and highly efficient labelling of living cells. These dyes are well suited for identifying the location of cells within a population and monitoring the dynamic interactions between cells in mixed cultures. WebSARTORIUS CORPORATION Incucyte Cytolight Rapid Dye for Live-cell Cytoplasmic Labeling, Red Manufacturer: SARTORIUS CORPORATION 4706 Supplied as dry … WebTo discriminate effector cells from target cells, effector cells were labeled with CTV, as per the manufacturer’s instructions, prior to the co-culture assays. For live-cell IncuCyte imaging cytotoxicity assay, effector cells were labeled with CytoLight rapid green (Sartorius, Göttingen, Germany), as per the manufacturer’s instructions. easy fried tofu recipe