Click-it rna imaging kits invitrogen c10329
WebDec 13, 2024 · B30756 Shop Invitrogen™ Click-iT™ RNA Alexa Fluor™ 488 Imaging Kit at Fishersci.co.uk Invitrogen™ Click-iT™ RNA Alexa Fluor™ 488 Imaging Kit 1 kit … WebOct 19, 2024 · The nascent RNA was visualized using the Click-iT RNA Alexa Fluor 488 Imaging Kit (Molecular Probes, Invitrogen, C10329). Mounting was done using ProLong Diamond Antifade Mountant with DAPI (P36962, Life Technologies). 3i CSU-W1 Spinning disk (objective 40×, 63× or 100×) microscope was used for obtaining images.
Click-it rna imaging kits invitrogen c10329
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WebCatalog Number C10329 This product is no longer available on Biocompare. Biocompare is the leading resource for up-to-date product information, product reviews, and new … WebOct 5, 2024 · For detection of nascent RNA, cells were incubated with 1 mM EU 30 min before fixation and EU detection was performed using Click-iT RNA imaging kit (Invitrogen, C10329) according to manufacturer ...
WebApr 2, 2024 · Click-iT RNA Imaging Kit: Invitrogen: Cat#C10329: Click-iT RNA Capture Kit: Invitrogen: Cat#C10365: NEBNext ChIP-Seq kit: NEB: Cat#NEB6240S: NEBNext Ultra Directional RNA Library Prep Kit: NEB: ... (Invitrogen, C10329). Alexa Fluor 488 azide was employed in the Click-iT reaction performed with fixed cells previously incubated with or … WebMillipore pig rabbit millipore sigma sab4500548 Pig Rabbit Millipore Sigma Sab4500548, supplied by Millipore, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations.
WebCatalog number: C10365. The Click-iT™ Nascent RNA Capture Kit, uses our proprietary Click-iT™ chemistry to capture with high efficiency and sensitivity newly minted RNAs. … WebNov 28, 2024 · Embryos were cultured in KSOM medium with 100 μM 5-ethynyl uridine (EU) for 2 h. Fixation, permeabilization, and staining were performed using the Click-iT ® RNA Alexa Fluor ® 488 Imaging Kit (Thermo, 48 C10329) according to the manufacturer's protocol. Imaging of embryos was performed on a Zeiss LSM710 confocal microscope. …
WebSimilar to BRD4, we observed that H4K12ac occupancy increases near the transcription start sites (TSS) of estrogen-induced genes as well as at distal ERα binding sites in an estrogen-dependent manner. Interestingly, H4K12ac occupancy highly correlates with BRD4 binding and enhancer RNA production on ERα-positive enhancers. Consistent with an i...
WebMay 25, 2024 · Alexa Fluor 488 goat anti-rabbit IgG (Invitrogen, A11034). 4. Alexa Fluor 594 goat anti-mouse IgG (Invitrogen, A11032). 2.5 Nascent Transcription Analysis. 1. Click-iT RNA Imaging Kit (Invitrogen, Cat No. C10330)* C10329 labels EU with Alexa Fluor 488. C10330 labels EU with Alexa Fluor 594. how can i find out my motoring convictionsWebThe Click-iT EdU Alexa Fluor 555 Imaging Kit provides a superior alternative to BrdU assays for measuring cell proliferation. EdU (5-ethynyl-2'-deoxyuridine) is a nucleoside analog of thymidine and is incorporated into DNA during active DNA synthesis. With Click-iT EdU, mild fixation and detergent permeabilization is sufficient for the small ... how can i find out my blood group ukWebCitations & References. The Click-iT™ RNA Alexa Fluor™ 488 Imaging Kit enables detection of global RNA transcription temporally and spatially in cells and tissues (PNAS … The Click-iT™ RNA Alexa Fluor™ 488 Imaging Kit enables detection of global … TaqMan Real-Time PCR Assays. Antibodies. Oligos, Primers & Probes how many people born every dayWebApr 7, 2024 · with 10% fetal bovine serum (Life Technologies) in 5% CO. 2. at 37˚C. ARID1A was knocked. ... with Click-iT RNA Imaging Kits (Invitrogen, C10329) using Alexa Fluor 488 dye according to. how many people born every second in americaWeb(C10329) Click-iT™ RNA Alexa Fluor™ 488 Imaging Kit - Thermo Scientific - CiteAb. Kit [C10329] Click-iT™ RNA Alexa Fluor™ 488 Imaging Kit by Invitrogen Antibodies. Menu Sign in or Register Search; Custom Suppliers; Data; Citations; Images; Listing; Contact; ... Life Technologies, Pierce, Gibco, Applied Biosystems, Affymetrix ... how can i find out my travel historyWebOct 8, 2024 · Exosome-packaged RNA labeling was performed using a Click-iT RNA Imaging Kit according to the manufacturer’s instructions (C10329, Invitrogen). Briefly, CAFs were labeled with 100 μM 5-EU for 24 h, and LUAD cells were labeled with Dil for 10 min. Both cells types were then washed and co-cultured for 8 or 24 h. how many people born in 1911 are still aliveWebClick-iT® RNA Imaging Kits Catalog nos. C10329, C10330 Introduction The Click-iT® RNA Imaging Assay enables detection of global RNA synthesis temporally and spatially … how many people born in 1919 are still alive