Biotin apc

WebJun 12, 2024 · The resulting phage supernatants were incubated with 100 nM (first round), 50 nM (second round), or 10 nM (third round) biotin–APC in 1 ml of DPBS/3% BSA/0.05% TWEEN 20/1 mM CaCl 2 for 2 h at RT ... WebAnti-Biotin Antibody Products. The following lists a number of Biotin antibodies from various sources. The target antigen refers to the small molecule vitamin, biotin (also known as vitamin H). Biotinylated proteins are covalently linked to biotin for detection or isolation using avidin or streptavidin. Antibodies against the biotin label can ...

Anti-Biotin Antibodies Invitrogen - Thermo Fisher Scientific

WebCF® Dye, biotin, and FITC conjugates of Annexin V are provided as 50 ug/mL solutions in 10 mM Tris, 1 mM EDTA, 30 mM NaCl, 1 mg/mL bovine serum albumin, 0.1% sodium azide, pH 7.5. R-PE and APC Annexin V conjugates are supplied in PBS, 5 mg/mL BSA, 0.05% sodium azide at 5 uL per test. WebAPC-streptavidin conjugate comprises streptavidin (as the biotin-binding protein) with APC covalently attached (as the fluorescent label). It is commonly used as a second step reagent for indirect immunofluorescent staining, when used in conjunction with biotinylated primary antibodies. Cat No. 16902 list of australian olympic swimmers https://robina-int.com

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WebStreptavidin Phycoerythrin (RPE), APC, & Tandem Conjugates. Streptavidin phycoerythrin, also known as SA-PE or R-PE streptavidin, is typically used for flow cytometry, microarrays, ELISA, and other applications that require either high sensitivity or simultaneous multicolor detection. Phycoerythrin is a member of a family of proteins … WebVimentin is a developmentally regulated intermediate filament protein (IFP) found in cells of mesenchymal origin. It is believed to be involved with the intracellular transport of proteins between the nucleus and plasma membrane. Unlike other IFP proteins, vimentin is expressed, along with desmin, during the early stages of cellular development ... WebBiotin Antibodies. Antibodies that detect Biotin can be used in several scientific applications, including Western Blot, Immunocytochemistry, ELISA, Immunohistochemistry and Flow Cytometry. These antibodies target Biotin in Rat, Mouse and Human samples. Our Biotin polyclonal, monoclonal and recombinant monoclonal antibodies are … list of australian penny values

APC Monoclonal Antibody (eBioAPC-6A2), Biotin (13 …

Category:Analysis of Myeloid Cells in Mouse Tissues with Flow Cytometry

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Biotin apc

Cell-Surface Vimentin (CSV) Monoclonal Antibody (84-1), APC

WebThe APC003 antibody reacts with both allophycocyanin (APC) alone and APC conjugated with antibody or streptavidin, as well as APC tandem dyes such as APC/Cy5.5 and APC/Cy7. When APC003 antibody is used in a three-step staining procedure or separation of APC-antibody labelled cells, it neither quenches APC fluorescence nor reacts with … Streptavidin-APC is useful as a second step reagent for indirect immunofluorescent … Description. CD80 is a 60 kD highly glycosylated protein. It is a member of … WebJan 21, 2024 · Application Notes : Streptavidin-APC is useful as a second step reagent for indirect immunofluorescent staining, when used in conjunction with biotinylated primary antibodies. The average molecular weight of Streptavidin-APC is 320 kD and Streptavidin alone is 52 kD. more info or order : BioLegend product webpage.

Biotin apc

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WebAbs/Em Maxima: 440/515 nm. Extinction coefficient: 40,000. Molecular weight: ~716. Excitation laser line: 405 nm. Replaces: Alexa Fluor® 430. Features. Photostable dyes suitable for microscopy. Perfect match for the CFP … WebItem Biotin Antibody, APC. Company Miltenyi Biotec. Price Pricing Info Supplier Page View Company Product Page. Catalog Number 130-113-850. Quantity 30 tests in 60 µL. Applications Flow cytometry. Conjugate/Tag APC. Format Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.

WebPolyclonal Antibody for studying APC. Cited in 35 publications. Validated for Western Blotting, Immunoprecipitation. ... Incubate membrane with Anti-rabbit IgG, HRP-linked Antibody (#7074 at 1:2000) and anti-biotin, HRP …

WebStreptavidin binds to biotin with high affinity. Streptavidin-APC is useful for detecting biotinylated antibodies. The excitation of APC by 600-635 nm laser light induces a fluorescence emission maximum of 660 nm. Formulation Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide. WebFast: One-step labeling procedure. It only requires 30 seconds hands on time, with an incubation time of either just 15 mins with the LYNX Rapid Plus Kits or 3 hours with the LYNX Rapid Kits to finish the conjugation. …

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WebJun 2, 2024 · APC is maximally excited at 652 nm (with a secondary maximum at 625 nm) and emits at 658 nm. Its fluorescence falls in the … images of old buckboard wagonsWeb(130-090-856) Anti-Biotin-APC monoclonal This product has been discontinued by Miltenyi Biotec, but remains here on CiteAb for record purposes. Supplier Miltenyi Biotec. Host Mouse Type Primary Clonality Monoclonal … list of australian prime ministers pdfWebProduct Specific Information. Description: The eBioAPC-6A2 monoclonal antibody reacts with allophycocyanin (APC) and APC-Cy7, with no crossreactivity to fluorescein (FITC) or phycoerythrin (PE). When using eBioAPC-6A2 as a second-step antibody, in combination with an appropriate third step, to bind a APC-conjugated primary antibody, there is no ... images of old buicksWebJul 4, 2024 · National Center for Biotechnology Information images of old cabins in the woodsWebJun 19, 2024 · APC-eFluor780: CD3e: eBioscience: 47-0031-82: 145-2C11: 1/200: 0.5 μL: eFluor450: CD19: eBioscience: 48-0193-82: eBio1D3 (1D3) 1/200: 0.5 μL: BV510: CD11c: Biolegend: ... use biotin-labelled F4/80 and PE-Cy7 conjugated streptavidin to amplify the signal. Problem 2. Red blood cell residue in the blood samples. Potential Solution. The … list of australian parkrunsWebMix-n-Stain™ Biotin Antibody Labeling Kit. From: $120 Sizes: 3x (5-20 ug) labelings, 5-20 ug labeling, 20-50 ug labeling, 50-100 ug labeling Catalog #: 92444 - 92286 View all. Label your antibody with biotin in just 15 minutes without a purification step. Labeling tolerates many common buffer components including BSA and ascites. images of old cars stuck in mudWebThe Anti-Biotin antibody does not bind to free biotin which is often present in cell culture media.Additional information: Clone RE746 displays … list of australian postcodes download